Faculty

Rotem Karni, PhD

Associate Professor of Genetics

Department: Genetics

Graduate Group Affiliations

Cell and Molecular Biology

Contact information

One Ucity square, floor 4, room 4018
Philadelphia, 19104

Office: 2158985073
Lab: 2158985072

Education
BsC
The Hebrew University of Jerusalem, 1997.
Ph.D. (Biological Chemistry)
The Hebrew University of Jerusalem, Israel., 2002.

Links
Lab webstite

Permanent link

> Perelman School of Medicine > Faculty > Details

Selected Publications

Lea Cohen-Attali, Yelena Mostinski, Guy Biber, Daniela Kaludjerski, Mor Hanan, Reut Eshel, Alexandra Polyansky, Igor Pery, Aric Orbach, Rotem Karni, Amir Mor: FTO regulates centrosome function and mitotic fidelity in cancers with chromosome instability (CIN). bioRxiv 2026.03.22.713449; doi: https://doi.org/10.64898/2026.03.22.713449 2026.

Shlomit Ezer, Shira Yanovsky-Dagan, Amit Granit, Matthew McDougal, Taeyoung Hwang, Israel Antman, Rotem Karni, Wan Hee Yoon, Ann Saada, Adi Inbal, Tamar Harel: Allele-specific antisense oligonucleotide treatment rescues atad3-associated phenotype in zebrafish. bioRxiv 2026.05.20.726050; doi: https://doi.org/10.64898/2026.05.20.726050 2026.

Shivang Khandelwal, Ela Elyada, Reut Japha, Manar Abu Diab, Anurag Prabhu, Zahava Siegfried and Rotem Karni.: Molecular characterization of the heterozygous loss of function mutations in the X-linked PCDH19 gene causing PCDH19-Cluster Epilepsy. bioRxiv 2026.03.16.712128; doi: https://doi.org/10.64898/2026.03.16.712128 2026 Notes: 2026.03.16 (uploaded).

Carla Azar-Koussa, Marina Sakran, Efraim Rahamim, Anurag V Prabhu, Samar Salem, Adina Heinberg, Zahava Siegfried, Meital Ben-David-Naim, Eran Zimran, Erez Levanon, Zvika Granot, and Rotem Karni: Targeting the 3’ splice site by a decoy oligonucleotide attenuates U2AF1 splicing activity and inhibits leukemia. BioRxiv doi: https://doi.org/10.64898/2026.06.30.735457 CSH Press, 2026.

Prabhu, A.V., Azar-Koussa, C., Siegfried, Z. and Karni R. : Spliced to kill: RNA mis-splicing derived cancer neoantigens. Trends Cancer. 11: 611-613, 2025.

Shay Tzaban, Priyanga Appasamy, Elad Zisman, Shiri Klein, Reyut Lewis, Houlin Yu, Akanksha Khorgade, Marc A. Schwartz, Moshe Sade-Feldman, Thomas Eisenhaure, Oren Parnas, Aron Popovtzer, Cyrille Cohen, Eric Shifrut, Aziz M. Al’Khafaji, Rotem karni, Galit Eisenberg, Nir Hacohen, Michal Lotem.: RNA splicing dynamics in CD8 T cells uncovers isoforms that impact T cell-mediated cancer immunotherapy. bioRxiv 2025 Notes: 2025.09.07.674706; doi: https://doi.org/10.1101/2025.09.07.674706.

Yehezkel Sztainberg, Maya David Teitelbaum, Ilana Buchumenski, Hagit Porath, Dror Sharon, Eyal Banin, Rotem Karni, Erez Y. Levanon, Ariel Feiglin.: The prevalent NR2E3 c.932G>A mutation induces aberrant splicing that can be rescued using splice-shifting antisense oligonucleotides. bioRxiv 2024 Notes: 2024.05.01.592034; doi: https://doi.org/10.1101/2024.05.01.592034.

Rosenberg-Mogilevsky, A., Siegfried, Z. and Karni R.: Generation of tumor neoantigens by RNA splicing perturbation. Trends Cancer. 11:12-24 11: 12-24, 2024.

Yu KM, Trimble DJ, Conner CR, Sheinberg DL, Low GMI, Saluja K, Schmitt KM, Karni RJ.: Oligometastatic Parathyroid Carcinoma Presenting as Neck Pain: A Case Report. Ear Nose Throat J 102(19): 635-639, Oct 2023.

Amar-Schwartz A, Cohen Y, Elhaj A, Ben-Hur V, Siegfried Z, Karni R, Dor T.: Inhibition of nonsense-mediated mRNA decay may improve stop codon read-through therapy for Duchenne muscular dystrophy. Hum Mol Genet 32(15): 2455-2463, Jul 2023.